Histology

Core Director: Patrick Mueller, Ph.D.

image of a cryostat machineThe Department of Physiology's Histology Core contains a ThermoFischer HM 550 Cryostat capable of sectioning tissue at various subzero temperatures.  Section thickness can be varied from single microns to several hundred microns.  The available Vacutome option keeps the cryostat free of contaminant tissue (important in RNA work) and allows for disposal of unwanted sections.  The Vacutome is also useful for reducing curling of tissue for immunohistochemsity image of the computer used for histologyor in situ hybridization studies, and is valuable for sectiong tissue for later laser capture microdissection.

The Histology Core has also made available for use a standard brightfield Nikon Eclipse Ci compound microscope with 2X, 10X, 20X, 40X objectives and a Spot Insight Color Camera for viewing and capturing digital images. 

Orientation and prior sign-up is required for use of all equipment and can be arrange through Toni Azar (tazar@med.wayne.edu) or Dr. Mueller (pmueller@med.wayne.edu).  Until further notice, equipment is available free of charge (minus own materials) to all Physiology personnel.  Costs to those individuals outside the Department will be assessed on a case by case basis by the Chair of Physiology, Dr. J.-P. Jin (jjin@med.wayne.edu).