Core Director: Xuequn Chen, Ph.D.

Potential applications of the Mass Spectrometry (MS) -based proteomic approach

The LC-MS instruments to conduct proteomics analyses include: 1). Dell computer system with Analyst and Bioanalyst software; 2). Dionex  nanoLC including Ultimate micropump, Switchos and Famos autosampler; 3). Protana Nanospray source with imaging system; 4). The QSTAR XL Hybrid LC-MS/MS System ideal for iTRAQ-based quantitative proteomics analysis;    5). Remote access to Mascot server in the Proteomics Core in the same building to conduct database searches for mass spec data:

• Identification of proteins (organellar proteome, interacting partners etc);
• Relative quantification of protein abundance with iTRAQ or other methods;
• Peptide mapping (high sequence coverage);
• Determination of intact protein molecular weight